The number of children and adolescents with chronic health conditions surviving into adulthood has dramatically increased over the last half-century. Individuals with Chronic Kidney Disease (CKD) are one population that has seen some of the biggest increases in children surviving into adulthood. Becoming an adult is a critical period of time, such that an improper transition may lead to poor health outcomes. The purpose of this study was to evaluate the relationship between psychosocial factors and healthcare transition for adolescents and young adults with CKD. In this IRB-approved study, validated transition measures and psychosocial scales were administered in pediatric and adult nephrology clinics at UNC Hospitals to patients with CKD. Family functioning and social support were the main psychosocial factors that influenced transition readiness. These findings can be used to guide interventions to improve transition readiness and self-management for adolescents and young adults with CKD.