There are currently over 20 different pharmacogenomic tests being used in the oncology field. However, only a few studies have been conducted regarding knowledge and attitudes towards pharmacogenomic testing among clinicians, in particular nursing. This descriptive study (guided by Rogers' Diffusion of Innovation Theory) utilizing descriptive statistics and variable selection methods was conducted with 368 oncology nurses in the state of North Carolina to identify and test key elements of Rogers' Diffusion of Innovation theory that play a role in the adoption of pharmacogenomic testing into the oncology practice by assessing oncology nurses knowledge, attitudes, and support for use of pharmacogenomic testing. Oncology nurses who participated in this study had a low perception of their knowledge of both genomics and pharmacogenomic testing based on their perceived knowledge. Additionally, attitudes towards pharmacogenomic testing were overall positive. Attitudes toward pharmacogenomic testing specifically related to oncology had a more favorable response than attitudes towards pharmacogenomic testing in general. Furthermore, the study revealed that oncology nurses in this study utilize pharmacogenomic testing information routinely in their nursing care.