The purpose of this study was to assess the prevalence of retained primitive reflexes in healthy preschool children in the Southeastern United States. Method: Twenty-one children between ages 3 and 5 years enrolled in the same preschool class participated in the study. A physical therapist tested each child for the asymmetrical tonic neck reflex (ATNR), symmetrical tonic neck reflex (STNR), and tonic labyrinthine reflex (TLR) and the prevalence of each reflex was determined for the children overall and for boys and girls separately. Results: Only one child study displayed no presence of retained primitive reflexes. Six children (29% of subjects) scored between 1 and 2 on all reflexes tested, and 14 children (50% of subjects) scored a 3 or 4 on at least one of the reflexes. The TLR (95% of subjects) was the most commonly retained reflex, followed by the right ATNR (86% of subjects), left ATNR (81% of subjects), and STNR (67% of subjects). The number of subjects with evidence of reflex retention was nearly identical for each reflex in both boys and girls. Conclusions: Because of the high prevalence of primitive reflex retention found in this study and in previous work, routine screening of primitive reflexes in preschool-age children may be prudent to identify children who may need early intervention in the form of reflex integration therapy.