The aim of this work was to investigate the regulatory mechanisms underlying production of the unique volatile compound sodorifen in different Serratia species. For this purpose, expression analyses of the biosynthetic gene cluster were performed and global regulatory pathways in the sodorifen producing strain S. plymuthica 4Rx13 inactivated. As a result, biosynthesis of sodorifen was found to be predominantly regulated on a transcriptional level and the obtained results gave rise to the speculation that it might be involved in stress response of the producing bacteria.