This thesis focusses on the establishment of a technological process chain for prototyping of electro-optical circuit boards (EOCB)-based sensing platforms for optical fluid analysis with optical waveguides integrated with microfluidic channels on various substrates. The feasibility of this technology is proved by four optofluidic demonstrators, whose operation principles are based on light absorption in a fluid during light’s free propagation in a microchannel, on evanescent field absorption, on partial back reflection at the waveguide end-faces, and on thermo-optic interferometric effects.