The present study examined 2287 brachyuran crab specimens collected in Yemen and material from previous collections partially stored in museums. The specimens were collected from 58 sites along the Yemeni coastal waters at Red Sea, Gulf of Aden, Arabian Sea and Socotra Islands. They were collected from different habitats ranging from sandy shore to mangrove flats and rocky habitats, including different depth zones from the supralittoral zone to 300 m water depth. The present study obtained the first CO1 sequences in the region for 95 brachyuran specimens that were collected from Gulf of Aden.