Exposure to air pollutants including polycyclic aromatic hydrocarbons (PAHs) was linked to acute and chronic health effects. Comprehensive evidence is still limited. Thus we developed robust and sensitive analytical methods to jointly investigate the interplay between the biomarkers of PAHs, oxidative stress, and health profiles. Moreover, we proposed a concept of non-invasively using travelers between locations of different particulate matter (PM) concentrations as a simple and cost-effective model to investigate acute health effects from exposure to air pollutants.