This thesis provides the first genomic tools and resources to enhance pikeperch's innovative farming, optimal domestication, and adaption into modern intensive aquaculture systems, including a high-quality chromosome-level assembly, reference transcriptome, and gene expression atlas. The pikeperch genome was also used as a reference for comparative genomics analyses and population genetics analyses in domesticated individuals to establish the landscape of genetic variations. These findings lay the foundation for addressing critical issues in genomics-informed pikeperch farming.