Optrodes usually represent a combination of optical fiber or waveguide and single or multiple microelectrodes. This thesis explaines fabrication process of fiber-based optrode consisting of tapered optical fiber with two deposited gold electrodes on its surface. Furthermore, design and construction of custom mechanical grinding setup, that enables fabrication of tapered fiber tips of good optical quality with various cone angles, represents an important part of this thesis. Detailed optical and electrical characterization of fabricated optrodes is also presented.