With STEM imaging, slices as thick as 700 nm can be imaged and tomograms can be generated by angling the sample from -75° to +75° and imaging every 1.5°. Gold beads were used to align all images. The tomogram shows that EF-C PNF do not disrupt the cell membrane and further confirms the invagination formation and the reduced distance between plasma and nuclear membranes. One example is shown for xy, yz and xz image.