During a survey on the identification of plant-parasitic nematodes of several potato fields in Iran, Ditylenchus weischeri was found from the rhizosphere of weeds in Markazi province. Nematodes were extracted from soil using the tray method and then fixed according to the standard protocols. The morphological characters of these nematodes agreed with the description of D. weischeri. In addition, molecular analysis of this sample using 18S rRNA and 28S rRNA gene sequences indicated 99.7-100% and 99.9-100% similarity, respectively, to those of several isolates of D. weischeri. The Iranian population formed a strongly supported clade with several isolates of D. weischeri in 18S and 28S rRNA gene trees. This is the first report of D. weischeri from Iran.