The Mexican cyst nematode, Globodera mexicana, was reported from wild solanaceous plants collected from several locations in Mexico. The analysis of COI, cytb, ITS rRNA and hsp90 gene sequences suggested that G. pallida and G. mexicana are sister species. In this study, 15 new COI, three new cytb, six new hsp90, and two new ITS rRNA gene sequences were provided. Maximal in traspecific COI gene sequence diversity for G. mexicana was estimated as 9.9%. Morphological description of G.mexicana from type and another location was also provided. Considering our current knowledge on findings of G. mexicana, it has been suggested that G. mexicana originated and diversified in Mexico and the cysts of its ancestor have dispersed to Mexico by passing through the northern Andes and Central America. Future studies of the genus Globodera should focus on increased sampling in these regions of Central and South America from wild solanaceous plants to reconstruct a more complete picture of phylogeography and evolution of this genus.