Aim: This work aimed to investigate the antiviral activity of two 1,4-disubstituted-1,2,3-triazole derivatives(1 and 2) against Chikungunya virus (CHIKV) replication. Materials & methods: Cytotoxicity was analyzedusing colorimetric assays and the antiviral potential was evaluated using plaque assays and computationaltools. Results: Compound 2 showed antiviral activity against CHIKV 181-25 in BHK-21 and Vero cells.Also, this compound presented a higher activity against CHIKV BRA/RJ/18 in Vero cells, like compound1. Compound 2 exhibited virucidal activity and inhibited virus entry while compound 1 inhibited virusrelease. Molecular docking suggested that these derivatives inhibit nsP1 protein while compound 1 mayalso target capsid protein. Conclusion: Both compounds exhibit promising antiviral activity against CHIKVby blocking different steps of virus replication.