(A) A Venn diagram of genes expressed in aHCFs (orange) and fHCFs (blue). (B) Heat map of genes exclusively expressed segregating fHCFs from aHCFs. (C) Correlation between replicates of independent fHCF and aHCF samples (fHCF replicates in blue box, aHCF in red box, and fHCF–aHCF cross-comparison in purple box). (D) Top 5 hits of both canonical pathways analysis and physiological system development and function for transcriptomes of both aHCFs and fHCFs, with p values determined by using Ingenuity Pathway Analysis (Qiagen GmbH, Hilden, Germany). (E) Heat map showing representative genes that are commonly expressed in both fHCFs and aHCFs, as well as genes exclusively expressed in each. fpkm = fragments per kilobase of exon per million fragments mapped; IL = interleukin; other abbreviations as shown in Figure 1.