RatesTools configuration files and additional datasets for Panthera and Neofelis de novo mutation analyses. Configuration files are named using the convention: species_reference_dataset.config. Two configuration files are provided for each of the Neofelis runs as the two siblings required different minimum genotype quality (GQ) cut-offs.Also provided is the list of lion y-mers and and 'mappable' genome assembly of the newly reported lion genome in FASTA format. The mappable genome consists of the assembly's haplotype 2 (including X chromosome) with the Y chromosome added.