This data set provides estimates of pre-deforestation aboveground live woody biomass (AGLB) at 30-m resolution for deforested areas of tropical America, tropical Africa, and tropical Asia for the year 2000. The biomass estimates are only for areas where deforestation occurred during the period 2000 through 2012. These estimates represent biomass loss over this time period and can be used to derive average annual carbon emissions from tropical deforestation.