This dataset includes estimates of gross primary production (GPP) for the conterminous U.S., for 2018-02-15 to 2021-10-15, based on measurements of solar-induced chlorophyll fluorescence from the TROPOspheric Monitoring Instrument (TROPOMI) on the Sentinel-5P satellite platform. GPP was estimated from rates of photosynthesis inferred from SIF using a linear model and ecosystem scaling factors from 102 AmeriFlux sites. Knowledge of the spatiotemporal patterns of GPP is necessary for understanding regional and global carbon budgets. Broad-scale estimates of GPP have typically relied upon carbon cycle models linking spatial patterns of vegetation with remotely sensed environmental data. SIF provides a means to directly estimate photosynthetic activity, and therefore, GPP. Recent deployments of satellite platforms that measure SIF provide near-real-time measurements and represent a breakthrough in measuring GPP on a global scale. Regular SIF measurements can detect spatially explicit ecosystem-level responses to climate events such as drought and flooding. This dataset includes spatially explicit estimates of GPP (g m-2 d-1), uncertainty in GPP, and related TROPOMI SIF measurements (mW m-2 sr-1 nm-1) at 500-m resolution. The data are provided in NetCDF format.