Time-course expression data of Agave americana for 15 samples collected with three biological replicates, including eight samples of the mature fourth fully expanded leaf collected at 3, 6, 9, 12, 15, 18, 21 and 24 hours after the beginning of the light period; three young leaf samples collected at diel time points of 6, 12 and 21 hours, respectively; and four non-leaf samples (i.e., meristem, rhizome, root or stem) collected at 3 hr after the beginning of the light period.