1 Citation 121 Views 13 Downloads
Mammalian axons are specialized for transmitting action potentials to
targets within the central and peripheral nervous system. A growing body
of evidence suggests that, besides signal conduction, axons play essential
roles in neural information processing, and their malfunctions are common
hallmarks of neurodegenerative diseases. The technologies available to
study axonal function and structure integrally limit the comprehension of
axon neurobiology. Complementary-metal-oxide-semiconductor (CMOS) -based
high-density microelectrode arrays (HD-MEAs) have been designed to record
extracellular action potentials from neuronal cultures and allow tracking
axonal signals across hundreds of microelectrodes. Thanks to a low-noise
CMOS-design, HD-MEAs enable detection of APs across entire arbors of
cortical axons. HD-MEAs provide noninvasive access to axonal action
potentials and impose no constraints on the duration of the recording
sessions. Here we present extracellular electrical activity recorded from
rat's primary neurons using CMOS-based HD-MEA system (MaxWell
Biosystems AG). Datasets comprise electrophysiological recordings obtained
from primary motor and cortical neurons. Recorded signals were up-sampled
to 200 kHz following the Whitaker-Shannon interpolation formula. Recorded
signals were spike-sorted and spike-trigger averaged across an entire
array (26400 electrodes). Such signal representation is referred to as
‘axonal electrical image’.
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