The dataset published here was used to measure a high resolution 3D wavefom of isolated and reactivated axonemes from Chlamydomonas reinhardtii. It was further used to show twist-torsion coupling in these axonemes.
The data is organized in six folders:
1) high resoluton 3D averaged waveform of isolated and reactivated axonemes from Chlamydomonas Reinhardtii.Data files (MATLAB and txt format) contain the 3D coordinates (along the 3D arc-length) of 32 axonemal shapes that comprise one beat cycle. A corresponding txt file describes the details of the dataset.
2) 3D waveforms of single isolated and reactivated axonemes from Chlamydomonas Reinhardtii.Data files (MATLAB and txt format) contain the 3D shapes of 17 individual axonemes obtained from defocused darkfield-microsopy images. A corresponding txt file describes the details of the dataset.
3) Image Raw Data of single isolated and reactivated axonemes used to reconstruct the 3D waveformMovie files (multi-layer tif) of reactivated axonemes imaged with defocused-darkfield-microscopy. A corresponding txt file describes the details of the dataset.
4) Calibration of defocused darkfield-microscopy. Data file (MATLAB) contains the relationship between the z-position relative to the focal plane and the full-width-at-half-maximum (FWHM) of the axoneme signal, measured normal to the centerline as well as the z-stack of imges (multi-layer tif) used to extract this relation. A corresponding txt file describes the details of the dataset.
5) Distance between gold nano paricle (GNP) and the axonemal centerline as a function of the beat cycleData file (MATLAB) contains 20 measurements of d_C (where d_C is the normal distance between the center position of the GNP and the axoneme centerline in 2D images) as a function of time. A corresponding txt file describes the details of the dataset.
6) Image Raw Data of single isolated and reactivated axonemes with attached GNPs used to measure d_C. Movie files (multi-layer tif) of reactivated axonemes with attached gold nano particles (GNPs) imaged with darkfield-microscopy. A corresponding txt file describes the details of the dataset.