The PAM matrix (Dayhoff et al., 1978) describes the probability that original amino acid
will be replaced by another amino acid over a defined evolutionary interval. The unit of
evolutionary divergence is defined as the interval in which 1% of the amino acids have been
changed between two sequences. The work uses PAM250, which assumes the occurrence of 250-
point mutations per 100 amino acids.
So, for the given the protein sequence GIVEQCCASVCSLYQLENYCN, A will be replaced
by 1 -3 0 1 -3 -1 0 5 -2 -3 -4 -2 -3 -5 0 1 0 -7 -5 -1 as shown in Figure 2.
will be replaced by another amino acid over a defined evolutionary interval. The unit of
evolutionary divergence is defined as the interval in which 1% of the amino acids have been
changed between two sequences. The work uses PAM250, which assumes the occurrence of 250-
point mutations per 100 amino acids.
So, for the given the protein sequence GIVEQCCASVCSLYQLENYCN, A will be replaced
by 1 -3 0 1 -3 -1 0 5 -2 -3 -4 -2 -3 -5 0 1 0 -7 -5 -1 as shown in Figure 2.