Diffraction images of ascorbate peroxidase (APX, PDB code: 7BI1). They were collected using X-ray free electron laser from SACLA at 10 keV with the serial femtosecond rotation crystallography (SF-ROX) method. The MX300-HS CCD detector was used to record images. APX crystals belong to P42212 space group with a=b=81.94, c=75.05 Å. 8904 images were collected from 147 crystals, and 8747 images were merged at 1.5 Å resolution using CrystFEL 0.6.3 in the published result. The experiment was carried out on BL2 EH3. Note that the first image in each run is unuseful due to a control problem. These images are included in this upload.