This is a dataset compiled from 117 sampling stream sites distributed across two neotropical biomes (rainforest and grassland). Dataset included single scaled vales of human land-use (agriculture, pasture, urbanization, and afforestation), scaled values of multifaceted biodiversity of fish, arthropods, and macrophytes (taxonomic richness, functional diversity[FDis], CWV and CWM of trait categories [recruitment and life-history, resource and habitat-use, and body size]), scaled values of local environmental predictors (stream site depth, water quality deterioration index, and sediment heterogeneity index), scaled values of climate predictors (temperature and precipitation), and log of animal biomass (fish and arthropod).