The report describes the status of 36 zoonotic agents, 5 alimentary diseases without zoonotic potential, and 5 agents from water. Of the 46 agents studied, 23 are bacterial, 11 are parasitic, 11 are viral and prions. Full Report of Zoonoses, Alimentary and Water-borne Infections in the Slovak Republic in 2021 is available only in the Slovak language. In the case of interest, feel free to contact us via the efsa.focalpoint@land.gov.sk.