FIGURE. 3. Neighbor-joining tree of Niphargus COI sequences from Luxembourg. This tree was generated using p-distances in MEGA X. Coloured boxes indicate the different species delineated by ASAP and correspond with the 28S haploweb. Niphargus aquilex MOTU B from Luxembourg had no COI sequence data, hence the COI sequence of individual UK1 from the neotype locality of Niphargus aquilex sensu stricto was included.