Fig. 1. Domain architecture of Anacardium occidentale chitinase (AoChi). (A) Graphical representation of AoChi domains (SP: signal peptide; GH19 CatD: GH19 catalytic domain). The catalytic residues of AoChi, Lys(K)128, Glu(E)150 and Tyr(Y)183, are highlighted. (B) Alignment of a stretch of 61 residues from the primary structure of AoChi (from His124 to Trp184), which contains the 3 catalytic residues, with corresponding segments from homologous proteins belonging to plant species from different families and orders (Table S5). Alignment columns are colored according to the ALSCRIPT Calcons convention, as implemented in ALINE (Bond and Schüttelkopf, 2009), using a predefined color scheme, which reflects the conservation of amino acid properties in each column (dark red: identical residues; white: dissimilar residues). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)