Fig. 2. Maximum likelihood (ML) tree showing the phylogenetic relationship of Anacardium occidentale chitinase with representative plant chitinases from classes I, II, IV, VI and VII. The evolutionary history of the sequences was inferred by using the ML method and Whelan and Goldman + Freq. model (Whelan and Goldman, 2001). The tree with the highest log likelihood (12837.58) is shown. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter = 1.5328)). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 3.91% sites). The percentage of trees in which the associated sequences clustered together, as determined by the bootstrap test (100 replicates), is shown next to the branches. This analysis involved 62 amino acid sequences, and the final dataset contained a total of 371 positions (Table S2 and Fig. S7). Evolutionary analyses were conducted in MEGA X (Kumar et al., 2018).