Fig. 6. Morphological alterations induced in Lasiodiplodia theobromae hyphae by Anacardium occidentale chitinase. SEM images of untreated mycelium(A-B), mycelium treated with 500 μg AoChi (C–I) and mycelium treated with 5 μg Carbendazim (J–M) are shown. The assays to evaluate the morphological alterations in L. theobromae grown in vitro in the presence of AoChi and acquisition of SEM images were performed as described in the Methods section. SEM images from fungus grown in the presence of water (A–B) or Carbendazim (J–M) were included for comparison.