Fig. 5. The validation of the expression amount of candidates, which might relate to the indican biosynthesis pathway, by qRT-PCR. a, Indican synthase (PtIGS) and the degradation enzyme (PtBGL); b, indole synthesis-related proteins; c, proteins that might relate to the indole oxidation; d, UDP-glucose biosynthesis enzymes and transport proteins that might relate to the indican biosynthesis pathway. Each error bar shows the standard error. The detailed values of (a) and (b) are indicated in Supplementary Table 4S. Those values of (c) and (d) are showed in Tables 4 and 5, respectively.