At a constant nematic elasticity (flagella concentration) and confinement (H=100μm), higher KSA (120nM) or motor concentrations induce robust active stress that disrupts nematic alignment, resulting in chaotic behavior. Conversely, lower KSA (<60nM) concentrations fail to overcome the nematic elastic background's alignment, leading to a unique state where active microtubule bundles phase-separate into lanes within a uniform flagella background. The confocal micrograph reveals this distinct phenomenon, showing uniform flagella distribution alongside microtubule segregation into lane-like structures within the channel's limited confinement.