Showing the effect of E2 on the mRNA levels of SMC-specific markers in terminally differentiated SMCs. Terminally differentiated SMCs were derived from H9-ESCs. Expression levels of SMA-α, SM-22α, and smoothelin did not change significantly in the presence or absence of E2. Data analyzed by ANOVA followed by Tukey post-hoc test. Data shown represent the mean ± SD from three independent experiments, each performed in duplicate. (PPTX 86 kb)