Showing sex differences in the expression of MMP-2 and TIMP-1 proteins in hPSC-derived pSMCs. (A), (B), (C) Gelatin zymography of MMP activities in concentrated condition media from HuF3-pSMCs, H1-pSMCs, and H9-pSMCs cocultured with different concentrations of 17β-estradiol. Graphs show the densitometric data of active-MMP-2 and/or pro-MMP-2 activities. (D), (E) Western blot analysis of TIMP-1 in the concentrated supernatants from male H1-pSMC and female H9-pSMCs. *p < 0.05, **p < 0.01, compared to untreated cells; data analyzed by two-way ANOVA followed by Tukey post-hoc test. Data shown represent the mean ± SD from three independent experiments, each performed in triplicate. (PPTX 415 kb)