Supplemental Video legends Supplemental Video S1. Optical sectioning (z-stacks) of a fixed MD-GFP mouse kidney tissue section (left panel), freshly dissected and in vitro microperfused JGA preparation from a MD-GFP mouse (middle panel), and intact living MD-GFP mouse kidney in vivo (right panel) showing a MD plaque with 1-2 single, membrane-targeted eGFP-labeled MD cell (green), and the adjacent eGFP- (tdTomato+) cells of the MD plaque and glomerulus (red). All mice were treated with combined low-salt diet and ACE inhibition. MD cell membrane features, such as the basal cell major and minor processes can be visualized projecting towards the glomerulus and other MD cells. Nuclei were labeled with DAPI (blue). Supplemental Video S2. Representative images of MD cell minor processes. Optical sectioning (z-stack) of living, single, membrane-targeted eGFP-labeled MD cells (green), and the adjacent eGFP- (tdTomato+) cells of the MD plaque and glomerulus (red). Note the long, thin, hairlike minor cell processes, and their variable density in male (none or only a few) and female mice (numerous) in control condition, and after combined low-salt diet with ACE inhibition. Supplemental Video S3. Dynamic outgrowth of MD cell minor processes. High temporal and spatial resolution time-lapse recording of living MD cells illuminated by membrane-targeted eGFP (green), and the rapid outgrowth of a long basal process. Video recording is 2 min long. Supplemental Video S4. Rapid movement of vesicles within MD cell minor processes. High magnification image of a freshly isolated, living, eGFP-labeled single MD cell and it’s maculapodia network. Multiple vesicles are visible within the processes rapidly moving between the cell body and their tip in both directions (high GFP intensity). Video recording is 15 s long. Supplemental Video S5. Representative rotating 3D image of a whole-mount optically cleared kidney tissue from a female MD-GFP mouse receiving low salt diet and ACE inhibition using CLARITY. MD cells were identified with GFP labeling (green), and the basement membrane was labeled with collagen IV immunostaining (magenta). Cell nuclei were labeled with DAPI (blue). 3D surface rendering revealed the heterogeneous network of MD cell major and minor processes, running towards the AA, mesangium, and the EA. Supplemental Video S6. 3D reconstruction wit...