Additional file 4: Online Resource 4. Apparent phagocytosis of α-synuclein (+) neurons by microglia but not by astrocytes. Double immunofluorescence against α-synuclein (red) and Iba1 (green) or GFAP (green). Panels a and c are panoramic views. Panels b and d are orthogonal projections from 1-μm z-confocal optical sections that correspond to the top left and bottom right panels. The top right panels are the integrated image, and the bottom left panels are a horizontal optical Z-section. Yellow arrows show microglia with α-synuclein aggregation. The scale bar = 50 μm.