Additional file 1: Figure S1. Study design for protocol 1 and 2. In Protocol 1, 6 Sham and 68 MI mice were used. Sham mice were evaluated at day 14 postsurgery. MI mice were randomly assigned to 4 groups after the surgery for sacrifice on day 1 (n = 6), day 3 (n = 12), day 7 (n = 25), and day 14 (n = 25), and we evaluated a total of 24 MI mice (n = 6 each) that survived. In Protocol 2, the Sham mice (n = 24) were randomly assigned after surgery for sacrifice on day 1, 3, 7, 14 (n = 6 each). The MI mice (n = 52) were randomly divided into 2 groups just after the surgery for treatment with Lis for 2 weeks (MI + Lis, n = 29), or without Lis treatment (MI + Veh, n = 23). The MI + Veh mice were sacrificed on day 14. The MI + Lis mice were randomly assigned to 4 more groups for sacrifice on day 1 (n = 6), day 3 (n = 7), day 7 (n = 8), and day 14 (n = 8), and we evaluated a total of 24 MI + Lis mice (n = 6 each). MI, myocardial infarction; Veh, vehicle; Lis, lisinopril. Figure S2. Survival curves of Sham +Veh, MI + Veh, and MI + Lis mice at 14 days post-surgery. MI, myocardial infarction; Veh, vehicle; Lis, lisinopril (n = 6–23).