Copyright information:Taken from "Efficacy of Wnt-1 monoclonal antibody in sarcoma cells"BMC Cancer 2005;5():53-53.Published online 24 May 2005PMCID:PMC1164405.Copyright © 2005 Mikami et al; licensee BioMed Central Ltd.5% Crystal Violet staining of fresh primary cultures of metastatic osteosarcoma after the monoclonal anti-Wnt-1 antibody treatment. The concentration of both control and monoclonal anti-Wnt-1 antibodies used was 10 μg/ml. The monoclonal anti-Wnt-1 antibody also induced cell death in these fresh samples.