Additional file 2. Neither reactive oxygen species nor Syk are involved in TNF production/release by Cg-stimulated macrophages. (A, B) Peritoneal macrophages were pre-incubated with N-acetylcysteine (3 NAC - 3 mM) or with selective inhibitor of Syk (iSyk, 1, 3 μM - 30 min) and then stimulated with Cg (300 μg/ml). After 6 h, the supernatants were collected for TNF quantification by Elisa. Data are represent the mean ± SD of four independent experiments compared WT (Cg) vs Treatments groups to determine the level of statistical significance (ns, not significant).