Additional file 3. Cg-induced TNF released by macrophages did not involved Casp1/11 neither Inflammasome platform. (A) Peritoneal macrophages were pre-incubated with selective inhibitor of Casp1 (Z-YVAD; 25, 50 μM - 30 min) and then stimulated with Cg (300 μg/ml). After indicated times, the supernatants were collected for TNF quantification by Elisa. (B, C) Peritoneal macrophages harvested from naive WT, Casp1 1-/−, Casp1/11−/−, Casp11Tg, Nlrp3−/−, Nlrc4−/−, Pycard−/− mice were stimulated with Cg (300 μg/ml) or medium. After indicated time points, the supernatants were collected for quantification of TNF by Elisa. Data are represent the mean ± SD of four independent experiments compared WT vs Knockout/Treatments groups to determine the level of statistical significance (ns, not significant).