Additional file 10: Fig. S10. Gene and protein expression of Phospho1 mRNA and PHOSPHO1 protein in murine tissue (a) RT-qPCR of Phospho1 in murine tissues, high expression was seen in the gonad and brown adipose tissue (BAT) (b) Protein expression of PHOSPHO1 was detectable by western blot in the calvaria and bone. Non-specific binding of the PHOSPHO1 antibody was observed in the pancreas, seen in both WT and Phospho1−/− pancreatic tissue.