Summary
This metadata record provides details of the data supporting the claims of the related article: “Genetic interactions among Brca1, Brca2, Palb2 and Trp53 in mammary tumor development”. The related study conducted parallel conditional knockout (CKO) of Brca1, Palb2 and Brca2, individually and in combination, along with one copy of Trp53, in the mammary gland of nulliparous female mice in order to directly compare the latency and penetrance of tumour development associated with each gene and the histopathological and genomic features of the mutant tumours in the same setting. Type of data: whole exome sequencing, analyses of mouse mammary glands and cultured human cells; flow cytometry Subject of data: mouse of mixed background; DAOY cells from ATCC Sample size: Number of mice used (n=~25 per genotype) was determined empirically. Number of tumours sequenced (n=5 per host genotype) was determined empirically and by available financial resources. Data access The whole exome sequencing data are openly available in the Sequence Read Archive via the following accession: https://identifiers.org/ncbi/insdc.sra:SRP199480 (BioProject accession: PRJNA544737). Supplementary Table S1, which underlies Figure 1 and Table 1 of the related article, is shared openly with this data record in the Excel file ‘Table S1.xlsx’. Original blots underlying Figure 5 are also shared openly with this data record in the PDF files ‘Figure S4.pdf’ and ‘Figure S5.pdf’. Corresponding author(s) for this study Bing Xia, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08903. Phone: +1 732-235-7410; Email: xiabi@cinj.rutgers.edu Britta Weigelt, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Phone: +1 212-639-2332; Email: weigeltb@mskcc.org
Ethics oversight IACUC of Rutgers Robert Wood Johnson Medical School
This metadata record provides details of the data supporting the claims of the related article: “Genetic interactions among Brca1, Brca2, Palb2 and Trp53 in mammary tumor development”. The related study conducted parallel conditional knockout (CKO) of Brca1, Palb2 and Brca2, individually and in combination, along with one copy of Trp53, in the mammary gland of nulliparous female mice in order to directly compare the latency and penetrance of tumour development associated with each gene and the histopathological and genomic features of the mutant tumours in the same setting. Type of data: whole exome sequencing, analyses of mouse mammary glands and cultured human cells; flow cytometry Subject of data: mouse of mixed background; DAOY cells from ATCC Sample size: Number of mice used (n=~25 per genotype) was determined empirically. Number of tumours sequenced (n=5 per host genotype) was determined empirically and by available financial resources. Data access The whole exome sequencing data are openly available in the Sequence Read Archive via the following accession: https://identifiers.org/ncbi/insdc.sra:SRP199480 (BioProject accession: PRJNA544737). Supplementary Table S1, which underlies Figure 1 and Table 1 of the related article, is shared openly with this data record in the Excel file ‘Table S1.xlsx’. Original blots underlying Figure 5 are also shared openly with this data record in the PDF files ‘Figure S4.pdf’ and ‘Figure S5.pdf’. Corresponding author(s) for this study Bing Xia, Rutgers Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08903. Phone: +1 732-235-7410; Email: xiabi@cinj.rutgers.edu Britta Weigelt, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Phone: +1 212-639-2332; Email: weigeltb@mskcc.org
Ethics oversight IACUC of Rutgers Robert Wood Johnson Medical School