Additional file 7. Figure S6 Signalling effectors affected by DN R-Ras induction. (A) Expression of induced DN R-Ras ST88-14 MPNST cells compared to control, either uninduced DN R-Ras ST88-14 MPNST cells or induced GFP ST88-14 MPNST cells. R-Ras and R-Ras2 immunoblotting show the presence of the HA-tagged DN R-Ras as a thick band above the endogenous Ras/Ras2 levels. Immunoblots comparing the effect of DN R-Ras expression on cofilin, RhoA, pERK1/2, total ERK1/2, pAKT and total AKT are also shown. (B) Raf-1 RBD pulldown assays demonstrating the effect of DN R-Ras expression on the activation of R-Ras proteins.