Copyright information:Taken from "Relaxin family peptide receptors Rxfp1 and Rxfp2: mapping of the mRNA and protein distribution in the reproductive tract of the male rat"http://www.rbej.com/content/5/1/29Reproductive biology and endocrinology : RB&E 2007;5():29-29.Published online 10 Jul 2007PMCID:PMC1947996. Immunohistochemistry for Rxfp1 (1:100 dilution of primary antibody) was done in cryosections of the testis and vas deferens; sections were counterstained with methylene blue. Inserts show controls incubated with non-immune rabbit serum instead of primary antibody. Scale bars are 5 μm. Results are representative of at least 3 independent determinations, each with a different animal. ep = epithelial layer, in = interstitium, lu = lumen, m = muscular layer, sp = spermatid. Arrows indicate labeled epithelial cells, arrow heads indicate labeled muscular cells, and double-headed arrows indicate labeled spermatids.