Copyright information:Taken from "Relaxin family peptide receptors Rxfp1 and Rxfp2: mapping of the mRNA and protein distribution in the reproductive tract of the male rat"http://www.rbej.com/content/5/1/29Reproductive biology and endocrinology : RB&E 2007;5():29-29.Published online 10 Jul 2007PMCID:PMC1947996. Receptors were labeled red with immunofluorescent antibody against intracellular domains of the receptors, nuclei were labeled blue with DAPI. A and D: assay with permeabilized cells in the absence of the primary antibody. B and E: assay with non-permeabilized cells in the presence of the primary antibody. C and F: assay with permeabilized cells in the presence of the primary antibody. Rxfp1 antibody dilution 1:300, Rxfp2 antibody 1:100. Results are representative of 3 different cell cultures.