Copyright information:Taken from "Relaxin family peptide receptors Rxfp1 and Rxfp2: mapping of the mRNA and protein distribution in the reproductive tract of the male rat"http://www.rbej.com/content/5/1/29Reproductive biology and endocrinology : RB&E 2007;5():29-29.Published online 10 Jul 2007PMCID:PMC1947996. [A] Effects of relaxin (RLX, 0.5 μg/ml) and forskolin (FKL, 50 μM) on spontaneous contractions of the vas deferens from castrated rats. [B] Effect of pre-treatment for 30 min with RLX (0.5 μg/ml) or FKL (50 μM) on contractions of the vas deferens induced by 2.5 μM noradrenaline; * < 0.05 compared to relaxin (Student's test). [C] Effect of RLX (0.5 μg/ml), FKL (50 μM) and IBMX (500 μM) on contractions of the vas deferens induced by incubation in a depolarizing solution; *< 0.05 compared to relaxin (Newman Keuls). [D] Control experiment showing the relaxing effect of relaxin (RLX 0.25 μg/ml) in rat uterus. All experimental protocols were repeated at least 3 times with different animals.