Copyright information:Taken from "Relaxin family peptide receptors Rxfp1 and Rxfp2: mapping of the mRNA and protein distribution in the reproductive tract of the male rat"http://www.rbej.com/content/5/1/29Reproductive biology and endocrinology : RB&E 2007;5():29-29.Published online 10 Jul 2007PMCID:PMC1947996. Slices of the vas deferens were incubated for 10 min with PBS (control), RLX (5 μg/ml) or FKL (50 μM) and the cyclic AMP production was measured. Results are mean ± SEM of 3 independent experiments. * < 0.05 compared to control (Student's t test). [B] Northern blot analysis of the effect of relaxin on Mmp-7 mRNA levels in the vas deferens. Upper panel shows a representative autoradiogram with Mmp-7 and Gapdh mRNA after various periods of incubation with relaxin (1 μg/mL). Bottom panel shows densitometric analysis of two independent experiments. Error bars indicate SEM.