Additional File 1: Fig. S1. Characterisation of bovine PSCdMs. (A) Bright-field image of bovine PSCs grown on mitotically-inactivated MEFs. Bovine PSC colonies are circled in yellow. (B) RT-qPCR analysis comparing expression of pluripotency markers (NANOG and OCT4) and macrophage markers (CSR1R, PU.1 and RUNX1) in primary bovine PAMs 62 and bovine PSCdMs relative to bovine PSCs. Mean and SD of three technical replicates. (C) Bright-field and fluorescent images of bovine PSCdMs containing phagocytosed pHrodo beads 22 h after exposure.