channel 1 - Calcium-Bound Fura-red; channel 2 - DiOC6; channel 3 - Annexin-V; channel 4 - Calcium-Free Fura-red. Platelets of healthy donors were labelled with Fura-Red and added to the platelet-depleted WAS patient's blood. DiOC6 was added to stain granulocytes, Annexin-V was added to stain procoagulant platelets.