Additional file 3: Fig. S3. Frequency of IL-17+IL-6+ and IL-6+IFN-γ+ T cells expressing TLR2 and TLR4 in CCM patients. The mean frequency of dual (A) IL-17 and IL-6- or (B) IL-17 and IFN-γ-secreting (CD4+ and CD8+) T cells positive for TLR2 and TLR4 from asymptomatic (CCMAsympt, n = 14) and symptomatic (CCMSympt, n = 23) patients was determined before and after Pam3Csk4 (Pam3C, 1 μg/mL) or LPS (100 ηg/mL) addition. Data are shown as mean ± SD of seven independent experiments with 5 to 6 samples per experiment. Significance was calculated by comparing different cell culture conditions from CCMAsympt and CCMSympt groups, and the p values are > 0.05 (Kruskal–Wallis test and Dunn’s test).