Additional file 5: Figure S4. Effect of pyridoxal 5′-phosphate (PLP) addition time on GABA production. (A) B. adolescentis JCM 1275/pKKT427::POri-gadBC at initial pH of 4.4. (B) B. adolescentis JCM 1275/pKKT427::Pgap-gadBC at initial pH of 6.0. GABA (mM) is displayed as a solid black line, and glutamate/GABA conversion ratio is shown as a black separated line. Values are presented as the means ± SD. Analysis was performed using three independent bacterial cultures.