Additional file 1. Immunoblots representing subcellular distribution of C83 detected with the APP-CTF antibody in primary cortical cultures at 11 DIV. Fractions are represented with their loading controls: for fraction 1, cytosolic—GAPDH; for fraction 2, membranous—Na + /K + ATPase, and for fraction 3, nuclear—Histone 3. Cells were treated or not with DAPT (10 μM). AAV-C99 (right) indicates a positive control. WT cells were infected for 5 days with AAV-C99 and recovered at 11 DIV. Note that only C83 levels were detectable with DAPT treatment.